Biology Department

Andrea A. Duina, Ph.D.

Duina, Andrea

Andrea A. Duina, Ph.D.

Professor of Biology

Chair, Biochemistry/Molecular Biology Program

duina@hendrix.edu Office: Donald W. Reynolds Center 422 Research Lab: Donald W. Reynolds Center 423 (501) 450-4536 (501) 450-4547

Academic Background

  • B.S., University of Illinois-Champaign, 1992
  • Ph.D., Northwestern University, 1998
  • Postdoc, Harvard Medical School, 1998-2004

Research Interests

  • We use the budding yeast  Saccharomyces cerevisiae  to gain insights into the process of eukaryotic gene transcription.  We are particularly interested in the functional and physical interactions that occur between the histone chaperone complex yFACT and chromatin during active transcription.

Scientific Publications

  • A.A Duina and C.E. Turkal.  In press . Targeted in situ mutagenesis of histone genes in budding yeast.  J. Vis. Exp.
  • Johnson, P., Mitchell V., McClure K., Kellems M., Marshall S., Allison M.K., Lindley H., Nguyen H.-T.T., Tackett J.E., and A.A. Duina.  2015 .  A systematic mutational analysis of a histone H3 residue in budding yeast provides insights into chromatin dynamics.  G3: Genes, Genomes, Genetics 5, 741-749.

  • A.A. Duina, Miller M.E. and J.B. Keeney.  2014 .  Budding yeast for budding geneticists: a Primer on the Saccharomyces cerevisiae model system.  Genetics 197, 33-48. This article was highlighted in "Genetics Spotlight: A showcase of research and scholarship in selected articles from 2014" published by the Genetics Society of America.

  • Nguyen H.-T.T., Wharton W. II, Harper J.A., Dornhoffer J.R., and A.A. Duina.  2013 .  A nucleosomal region important for ensuring proper interactions between the transcription elongation factor Spt16 and transcribed genes in Saccharomyces cerevisiae.  G3: Genes, Genomes, Genetics 3, 929-940.
  • A.A. Duina.  2013 .  New roles for old characters:  an Educational Primer for use with "Vps factors are required for efficient transcription elongation in budding yeast".  Genetics 194, 27-33.
  • A.A. Duina.  2011 .  Histone Chaperones Spt6 and FACT:  similarities and differences in modes of action at transcribed genes.  Genet. Res. Int ., Article ID 625210.  This article is part of a Special Issue: Gene Control During Transcription Elongation.

  • Myers C.N., Berner G.B., Holthoff J.H., Martinez-Fonts K., Harper J.A., Alford S., Taylor M.N., and A.A. Duina. 2011 . Mutant Versions of the S. cerevisiae transcription elongation factor Spt16 define regions of Spt16 that functionally interact with histone H3. PLoS ONE 6, e20847.
  • Lloyd, A, K. Pratt, E. Siebrasse, M.D. Moran and A.A. Duina. 2009 . Uncoupling of the patterns of chromatin association of different transcription elongation factors by a histone H3 mutant in Saccharomyces cerevisiae . Eukaryot. Cell 8, 257-260.
  • Duina, A.A., A. Rufiange, J. Bracey, J. Hall, A. Nourani and F. Winston. 2007 . Evidence that the localization of the elongation factor Spt16 across transcribed genes is dependent upon histone H3 integrity in Saccharomyces cerevisiae . Genetics  177, 101-112.
  • Duina, A.A. and F. Winston. 2004 . Analysis of a mutant histone H3 that perturbs the association of Swi/Snf with chromatin. Mol. Cell. Biol.  24, 561-572.
  • Duina, A.A., H.M. Kalton, and R.F. Gaber. 1998 . Requirement for Hsp90 and a CyP-40-type cyclophilin in negative regulation of the heat shock response. J. Biol. Chem.  273,18974-18978.
  • Duina, A.A., J.A. Marsh, R.B. Kurtz, J.H.-C. Chang, S. Lindquist, and R.F. Gaber. 1998 . The peptidyl-prolyl isomerase domain of the CyP-40 cyclophilin homolog Cpr7 is not required to support growth or glucocorticoid receptor activity in Saccharomyces cerevisiae . J. Biol. Chem.  273, 10819-10822.
  • Duina, A.A., J.H.-C. Chang, J.A. Marsh, S. Lindquist, and R.F. Gaber. 1996 . A cyclophilin function in Hsp90-dependent signal transduction. Science  274, 1713-1715.
  • Duina, A.A., J.A. Marsh, and R.F. Gaber. 1996 . Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae , one of which is required for normal growth. Yeast  12, 943-952.

Other Publications

  • Duina, A.A. 2007. Listen to your calling. ASBMB Today (February 2007), 19-20.

Current Funding Support

  • National Science Foundation (2016-2019): "RUI: Uncovering mechanisms of Spt16-gene interactions". 

Recent Scientific Conferences: Organizer or Platform Session Chair

  • Platform Session co-Chair:  23rd Annual Southeastern Regional Yeast Meeting.  University of Alabama, Tuscaloosa, AL.  March 25-27, 2016.
  • Co-organizer :  22nd Annual Southeastern Regional Yeast Meeting. Heifer International Headquarters, Little Rock, AR.  March 13-15, 2015.

  • Platform Session Chair: 18th Annual Southeastern Regional Yeast Meeting.  Mississippi State University, Mississippi State, MS.  March 11-13, 2011.

  • Co-organizer:  17th Annual Southeastern Regional Yeast Meeting.  William J. Clinton Presidential Center, Little Rock, AR.  March 12-14, 2010.

Invited Seminars

  • Central Baptist College (AR) - Department of Math and Science. April 25, 2014.  Breaking chromatin to understand it: a genetic approach provides new insights into the transcription elongation process.

  • Rhodes College (TN) - Department of Biology. October 23, 2013.  Breaking chromatin to understand it: a genetic approach provides new insights into the transcription elongation process.
  • Arkansas INBRE Research Conference - Invited Speaker. October 18, 2013.  Identification of a Nucleosomal Region Important for Ensuring Proper Interactions between the Transcription Elongation Factor Spt 16 and Transcribed Genes in Budding Yeast.
  • Emory University School of Medicine (GA) - Department of Biochemistry, February 23, 2012.  Analysis of yeast cells expressing specific histone mutants provide new insights into the process of transcription elongation.
  • Louisiana State University Health Science Center, School of Medicine in Shreveport - Department of Biochemistry and Molecular Biology, January 20, 2011. Insights into the process of transcription elongation through the analysis of histone mutants in yeast.
  • University of Arkansas at Little Rock - Department of Applied Sciences, November 15, 2010.  Insights into the process of transcription elongation through the analysis of histone mutants in yeast.
  • University of Arkansas for Medical Sciences - Department of Biochemistry and Molecular Biology, September 15, 2010. New insights into the process of transcription elongation in yeast.
  • University of Iowa - Department of Biochemistry, December 11, 2008.  Insights into the mechanism of yFACT association across transcribed genes in vivo.
  • University of Central Arkansas - Department of Biology, February 18, 2008.  A role for histone H3 in ensuring proper distribution of the elongation complex yFACT across transcribed genes.
  • University of Arkansas at Fayetteville - Department of Biology, November 8, 2007. A role for histone H3 in ensuring proper distribution of the elongation complex yFACT across transcribed genes.
  • Rhodes College (TN) - Department of Biology, November 13, 2006. A dysfunctional relationship: when a transcription factor just can't let go.
  • University of Arkansas for Medical Sciences - Department of Physiology, September, 2005. Evidence that the localization of the elongation factor Spt16 across transcribed genes is dependent upon histone H3 integrity in yeast. 

Current Members of the Lab

  • Jessica Campbell (Undergraduate Student)
  • Michaela Edwards (Undergraduate Student)
  • Daniel Habenicht (Undergraduate Student)
  • Jennifer Harper (Laboratory Technician)
  • Graham Harris (Undergraduate Student)
  • Michelle Huynh (Undergraduate Student)
  • Sydney Ozerski (Undergraduate student)
  • Heather Prowse (Undergraduate Student)

Past Lab Members

  • Undergraduate Students  

    • Sarah Alford
    • Katy Allison
    • Ryan Banning
    • Jeffrey Biles
    • John Bracey
    • Brandon Cox
    • Alex Crocker
    • Jimmy Dornhoffer
    • Chengsi Gao
    • Jeffrey Hall
    • Jasmine Haller
    • Kelsie Holmes
    • Hunter Holthoff
    • Any Jivan
    • Paige Johnson
    • Martha Kellems
    • Alex Khalaf
    • Harrison Lindley
    • Amanda Lloyd
    • Lindsay Loftis
    • Kirby Martinez-Fonts
    • Catey May
    • Kelsi McClure
    • Taylor McElroy
    • Hina Metha
    • Ira Miller
    • Ginny Mitchell
    • Meet Modi
    • Catherine Myers
    • John Neis
    • Trang Nguyen
    • Eugene Nymugenda
    • Malena Outhay
    • John Pennington
    • Jacob Pierce
    • Katie Pratt
    • Chaela Sickbert
    • Erica Siebrasse
    • Jessica Sweatt
    • Jessalyn Tackett
    • Claire Turkal
    • Hayley Warsinske
    • Lewis Wharton

    Laboratory Technicians

    • Gary Berner
    • Sarah Marshall
    • Landon Reeves
    • Kacey Sommers
    • Megan Taylor

     

     

     

Hendrix Biology Professor Receives NSF Grant

Dr. Andrea Duina awarded three-year $511,622 grant for research

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Hendrix Professor, Nine Students Publish Research

Dr. Andrea Duina and students publish research in G3 journal

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Professor Publishes Primer for Commonly Used Experimental Organism

Dr. Andrea Duina and peers pen primer for budding biologists

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Biology Professor Awarded NSF Grant

Dr. Andrea Duina receives $415,515 National Science Foundation grant

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