CONWAY, Ark. (February 13, 2017) – Hendrix College biology professor Dr. Andrea Duina and Claire Turkal ’18 recently published "Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast," a video article based on work from Duina’s research lab in the
Journal of Visualized Experiments (JoVE), a peer-reviewed scientific video journal.
Watch the video
here. The full article is available with a journal subscription
here.
From the
abstract:
“We describe a PCR- and homologous recombination-based system for generating targeted mutations in histone genes in budding yeast cells. The resulting mutant alleles reside at their endogenous genomic sites and no exogenous DNA sequences are left in the genome following the procedure. Since in haploid yeast cells each
of the four core histone proteins is encoded by two non-allelic genes with highly homologous open reading frames (ORFs), targeting mutagenesis specifically to one of two genes encoding a particular histone protein can be problematic. The strategy we describe here bypasses this problem by utilizing
sequences outside, rather than within, the ORF of the target genes for the homologous recombination step. Another feature of this system is that the regions of DNA driving the homologous recombination steps can be made to be very extensive, thus increasing the likelihood of successful integration
events. These features make this strategy particularly well-suited for histone gene mutagenesis, but can also be adapted for mutagenesis of other genes in the yeast genome.”
About
Hendrix College
Hendrix College is a private liberal arts college in Conway, Arkansas. Founded in 1876 and affiliated with the United Methodist Church since 1884, Hendrix is featured in Colleges
That Change Lives: 40 Schools That Will Change the Way You Think about Colleges and is nationally recognized in numerous college guides, lists, and rankings for academic quality, community, innovation, and value. For more information, visit
www.hendrix.edu.